《植物生理学报》 2016, 52(4): 436-444

花生响应水分胁迫过程中叶片AhCYP707A1蛋白和ABA分布的变化

曹家佳, 吕滟, 胡博^*, 李玲

华南师范大学生命科学学院, 广东省植物发育生物工程重点实验室, 广州510631

通信作者：胡；E-mail: hubo0610@126.com

摘　要：

CYP707A蛋白是高等植物内源ABA代谢主要途径的关键酶, 在水分胁迫过程中花生植株AhCYP707A1蛋白和ABA分布与水平的变化并不清楚。研究结果表明, 水分胁迫初期, 花生叶片AhCYP707A1蛋白表达均先被强烈抑制, 促进ABA积累, 随后蛋白表达回升, 参与调节ABA稳态; 叶片AhCYP707A1蛋白以及ABA主要分布在叶片维管组织中。水分胁迫下, 经CYP707A蛋白抑制剂处理后, 花生叶片ABA分布和含量均有提高。抗旱花生品种‘粤油7’在水分胁迫下, AhCYP707A1蛋白的表达以及分布均强于敏旱品种‘汕优523’, 表明ABA代谢更为活跃。

关键词：花生; 水分胁迫; AhCYP707A1蛋白; ABA分布

收稿：2015-11-12   修定：2016-03-03

资助：国家自然科学基金(31301247和31471422)和教育部高等学校博士学科点专项科研基金(20124407120008)。

Changers of AhCYP707A1 proteins and ABA distribution in peanut leaves under water stress

CAO Jia-Jia, LÜ Yan, HU Bo^*, LI Ling

Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China

Corresponding author: HU Bo; E-mail: hubo0610@126.com

Abstract:

CYP707A protein is the key enzyme in the major ABA catabolic pathway. It remains unclear that the change of AhCYP707A1 proteins and ABA distribution and level in leaves under water stress in peanut. The results revealed, the expression of AhCYP707A1 protein was decreased, which regulated the accumulation of ABA in peanut leaves under water stress. After that the expression of AhCYP707A1 protein was increased, and it was involved in regulation of ABA homeostasis. ABA and AhCYP707A1 were mainly distributed in the vascular cambium of peanut leaves. Under water stress, the distribution and content of ABA in peanut leaves were increased after the treatment with protein inhibitors of AhCYP707A. Drought-tolerant peanut cultivars showed higher levels of AhCYP707A1 expression and a stronger distribution in the vascular cambia with water stress treatment and which indicated that ABA metabolism of drought-tolerant peanut cultivars were more active.

Key words: peanut; water stress; AhCYP707A1; ABA distribution